Mar 22, 2024 · In this chapter, we provide a comprehensive review of the two primary methods for isolating insoluble tau, namely, sarkosyl and formic acid extraction (and their variants), which are …

Human control and AD postmortem brain tissues were homogenized and sedimented by ultracentrifugation in the presence of sarkosyl to enrich detergent-insoluble protein aggregates …

Jun 22, 2023 · Here, we demonstrated that tau isoforms differ between tauopathies in insoluble aggregates, but not in soluble brain extracts.

Preparation of sarkosyl-insoluble (SI) tau followed an adapted protocol used in our prior studies 19.

Jun 22, 2025 · Therefore, the aim of this study was to profile the sarkosyl soluble and insoluble proteomes in CBD, PiD, and PSP.

In this chapter, we provide a comprehensive review of the two primary methods for isolating insoluble tau, namely, sarkosyl and formic acid extraction (and their variants), which are employed for …

Sep 9, 2024 · While sarkosyl-insoluble tau seeds are produced uniquely after the exposure of cells to sarkosyl-insoluble AD SARK tau, PBS-extractable tau seeds are formed after injection of either AD …

Jan 9, 2012 · In this short chapter, we aim at reviewing the two main isolation methods, sarkosyl and formic acid extraction (and their variations), used for the biochemical isolation of insoluble tau in...

Dec 1, 2024 · In this study, we found that recombinant highly phosphorylated tau purified from NG108-15 rodent neuroblastoma/glioma cells transfected with both tau and GSK3β expression vectors …

Jan 30, 2024 · Our workflow uses sarkosyl fractionation to extract the pathological tau species from sample-limited brain specimens, followed by mass spectrometry (MS) to characterize and quantify …